primary 220 antibodies  (Cell Signaling Technology Inc)

Journal: Cellular Oncology (Dordrecht)

Article Title: YKL-40 protein expression in human tumor samples and human tumor cell line xenografts: implications for its use in tumor models

doi: 10.1007/s13402-021-00630-z

Figure Lengend Snippet: Representative images of YKL-40 immunostaining results on multi-tumor tissue microarray (TMA). Cancer cells were identified morphologically, and the staining intensities were assigned to four categories: 4 = high, 3 = intermediate-high, 2 = intermediate-low, 1 = low, and 0 = no staining. a Low magnification of whole tissue sample. Scale bar = 200 µm. b High magnification. Scale bar = 50 µm

Article Snippet: Directly afterwards, sections were incubated with an anti-YKL-40 primary monoclonal mouse antibody (MAb 201.F9, 2.1 mg/ml, IgG2b, epitope GAWRGTTGHHS, aa 210–220) diluted 1:100 in antibody diluent (#S0809, Dako, Carpinteria, CA, USA) for 60 min at RT.

Techniques: Immunostaining, Microarray, Staining

Journal: Cellular Oncology (Dordrecht)

Article Title: YKL-40 protein expression in human tumor samples and human tumor cell line xenografts: implications for its use in tumor models

doi: 10.1007/s13402-021-00630-z

Figure Lengend Snippet: Overview of YKL-40 protein expression in selected entities on multi-tumor TMA. Where applicable, subtypes were grouped and analyzed as one (see also Table )

Article Snippet: Directly afterwards, sections were incubated with an anti-YKL-40 primary monoclonal mouse antibody (MAb 201.F9, 2.1 mg/ml, IgG2b, epitope GAWRGTTGHHS, aa 210–220) diluted 1:100 in antibody diluent (#S0809, Dako, Carpinteria, CA, USA) for 60 min at RT.

Techniques: Expressing

Journal: Cellular Oncology (Dordrecht)

Article Title: YKL-40 protein expression in human tumor samples and human tumor cell line xenografts: implications for its use in tumor models

doi: 10.1007/s13402-021-00630-z

Figure Lengend Snippet: Representative YKL-40 immunostaining results of adjacent stroma in mouse xenograft tumor tissues (a–c) and human tumor samples (d–f) . Extracellular matrix components and stroma cells were identified morphologically. a Head and neck squamous cell cancer cell line UT-SCC-16A, b melanoma cell line FEMX-1, c pancreatic cancer cell line BxPC-3, d breast cancer, phyllodes subtype, e squamous cell esophageal cancer, f squamous cell lung cancer. Arrowheads point to fine granular staining in extracellular matrix components (mouse xenograft tumor tissue, a–c ) or small nucleated cells residing in adjacent tumor stroma and surrounded by fine granular staining (human tumor samples, d–f ). Scale bars = 50 µm

Article Snippet: Directly afterwards, sections were incubated with an anti-YKL-40 primary monoclonal mouse antibody (MAb 201.F9, 2.1 mg/ml, IgG2b, epitope GAWRGTTGHHS, aa 210–220) diluted 1:100 in antibody diluent (#S0809, Dako, Carpinteria, CA, USA) for 60 min at RT.

Techniques: Immunostaining, Staining

Journal: Cellular Oncology (Dordrecht)

Article Title: YKL-40 protein expression in human tumor samples and human tumor cell line xenografts: implications for its use in tumor models

doi: 10.1007/s13402-021-00630-z

Figure Lengend Snippet: Differential expression of YKL-40 protein in selected tumor types and subtypes on multi-tumor TMA. a Thyroid cancer subtypes, b colorectal cancer, c gastric cancer, d ovarian cancer, e esophageal cancer and f cervical cancer

Article Snippet: Directly afterwards, sections were incubated with an anti-YKL-40 primary monoclonal mouse antibody (MAb 201.F9, 2.1 mg/ml, IgG2b, epitope GAWRGTTGHHS, aa 210–220) diluted 1:100 in antibody diluent (#S0809, Dako, Carpinteria, CA, USA) for 60 min at RT.

Techniques: Expressing

Journal: Cellular Oncology (Dordrecht)

Article Title: YKL-40 protein expression in human tumor samples and human tumor cell line xenografts: implications for its use in tumor models

doi: 10.1007/s13402-021-00630-z

Figure Lengend Snippet: Percentage of YKL-40 positive tumor samples of all tumor samples in a adenocarcinomas and b squamous cell carcinomas on multi-tumor TMA

Article Snippet: Directly afterwards, sections were incubated with an anti-YKL-40 primary monoclonal mouse antibody (MAb 201.F9, 2.1 mg/ml, IgG2b, epitope GAWRGTTGHHS, aa 210–220) diluted 1:100 in antibody diluent (#S0809, Dako, Carpinteria, CA, USA) for 60 min at RT.

Techniques:

Journal: Cellular Oncology (Dordrecht)

Article Title: YKL-40 protein expression in human tumor samples and human tumor cell line xenografts: implications for its use in tumor models

doi: 10.1007/s13402-021-00630-z

Figure Lengend Snippet: Representative YKL-40 immunostaining results in mouse xenograft tumors derived from different human cancer cell lines. Cancer cells were identified morphologically, and YKL-40 staining was assessed in areas of vital tumor tissue. a Pancreatic cancer cell lines, b head and neck squamous cell cancer cell lines, c neuroblastoma cell lines, d ovarian cancer cell lines, e colorectal cancer cell lines, f melanoma cell lines. Scale bars = 50 µm

Article Snippet: Directly afterwards, sections were incubated with an anti-YKL-40 primary monoclonal mouse antibody (MAb 201.F9, 2.1 mg/ml, IgG2b, epitope GAWRGTTGHHS, aa 210–220) diluted 1:100 in antibody diluent (#S0809, Dako, Carpinteria, CA, USA) for 60 min at RT.

Techniques: Immunostaining, Derivative Assay, Staining